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JoVE Journal
Biology

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代谢标记和流式细胞仪测定细胞周期进程的动力学
 
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代谢标记和流式细胞仪测定细胞周期进程的动力学

Article DOI: 10.3791/4045
May 22nd, 2012

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Summary May 22nd, 2012

Please note that all translations are automatically generated.

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在细胞周期各阶段的进展和动力学跟踪微妙的变化,可以实现通过碘化丙啶与BrdU和总DNA染色核酸代谢标记的组合使用。这种方法避免了化学循环细胞同步的需要,从而防止非特异性的DNA损伤,这反过来又影响细胞周期进程的介绍。

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