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Chapters
- 00:05Title
- 01:29Coupling Antibodies to MagPlex Microspheres, using the xMAP Antibody Coupling Kit
- 04:58Antibody Screening
- 07:20xMAP Functional Assay
- 09:07ELISA Assay
- 09:44Results: Comparison of an ELISA Assay for TNF-alpha Cytokine to the Converted Assay using the xMAP Platform
- 14:28Conclusion
Un ELISA può essere facilmente convertito in un saggio Luminex xMAP e, attraverso i benefici di multiplexing, diversi anticorpi possono essere proiettati contemporaneamente per identificare una coppia ottimale anticorpo, con conseguente aumento della sensibilità e gamma dinamica, riducendo i costi dosaggio.
Tags
ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications
