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Chapters
- 00:05Title
- 01:29Coupling Antibodies to MagPlex Microspheres, using the xMAP Antibody Coupling Kit
- 04:58Antibody Screening
- 07:20xMAP Functional Assay
- 09:07ELISA Assay
- 09:44Results: Comparison of an ELISA Assay for TNF-alpha Cytokine to the Converted Assay using the xMAP Platform
- 14:28Conclusion
En ELISA kan enkelt konverteres til en Luminex xMAP analysen og gjennom fordelene ved multipleksing, flere antistoffer kan være skjermet samtidig for å identifisere en optimal antistoff par, noe som resulterer i økt følsomhet og dynamisk omfang, og samtidig redusere analysen kostnad.
Tags
ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications
