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Chapters
- 00:00Title
- 00:16Introduction
- 01:58Preparation of Cell Suspension from Lymph Nodes
- 04:04Erythrocyte Lysis
- 06:13Magnetic Labelling of Cells and Setting up MACS Column
- 07:49Separation of CD4+ T cells using MACs Column
- 09:13Conclusion
Tags
CD4+ T CellsMouse Lymph NodesMiltenyi MACS PurificationCell IsolationMagnetic Bead-based Cell SeparationPositive SelectionNegative SelectionUntouched CD4+ Helper T CellsPurityProtocol2-Photon Imaging Of Peripheral Lymph Nodes In MiceAdoptive TransferFACS AnalysisAntibodiesNon-sterile MethodSterile Conditions
