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突触荧光自动定量<em> C。线虫</em
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JoVE Journal Neuroscience
Automated Quantification of Synaptic Fluorescence in C. elegans
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突触荧光自动定量<em> C。线虫</em

DOI:
10.3791/4090-v

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12:22 min

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August 10, 2012

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1Department of Biological Sciences,University of Toledo

Chapters

  • 00:05Title
  • 01:29Preparation of Worms for Imaging
  • 04:50Confocal Imaging
  • 07:34Automated Identification and Analysis of Individual Synaptic Clusters
  • 10:28Representative Results
  • 11:58Conclusion

Summary

Automatic Translation

Automatic Translation

强烈的大量聚集在突触的神经递质受体影响突触强度。这种方法量化单突触决议的荧光标记的神经递质受体在三个层面<em> C。线虫</em>，让数百个突触被迅速的特点是在一个单一的样本没有推出Z-平面上的投影的扭曲。

Tags

Automated QuantificationSynaptic FluorescenceC. ElegansSynapse StrengthNeurotransmitter ReceptorsSynaptic SitesPostsynaptic MembraneReceptor TraffickingSynaptic PlasticityNeuromodulationFluorescence MicroscopySynaptically-localized Neurotransmitter Receptor AbundanceSynaptic DevelopmentSynaptic PlasticityCaenorhabditis ElegansNative SynapsesAutomated IdentificationThree Dimensions

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