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Behandling af primær hjernetumor Tissue for stamcelle Analyser og Flow Sortering
JoVE Journal
Medicine
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JoVE Journal Medicine
Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
DOI:

08:14 min

September 25, 2012

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Chapters

  • 00:05Title
  • 01:30Culture of Brain Tumor Tissue
  • 03:21Tumorsphere Dissociation for Flow Cytometry
  • 04:36Surface Staining
  • 05:17Flow Cytometry Acquisition and Analysis
  • 06:00Self-renewal Assay
  • 06:26Single Cell RT-PCR
  • 07:08Results: Dissociated Brain Tumor Tissue Can Be Sorted for Stem Cell Assays
  • 07:42Conclusion

Summary

Automatic Translation

Identifikationen af ​​hjernesvulst initierende celler (BTICs), de sjældne celler i en heterogene tumor besidder stamceller egenskaber, giver ny indsigt i den menneskelige hjerne tumor patogenese. Vi har forfinet særlige dyrkningsbetingelser at berige for BTICs, og vi rutinemæssigt bruger flowcytometri til yderligere berige disse befolkningsgrupper. Selvfornyelse assays og transkript analyse ved enkelt celle RT-PCR kan efterfølgende udføres på disse isolerede celler.

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