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定量测量的侵袭伪足介导的细胞外基质蛋白水解单和多细胞的情况下
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
DOI:

14:23 min

August 27, 2012

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Chapters

  • 00:05Title
  • 01:21Production of Oregon Green 488-gelatin Coated Coverslips
  • 04:48Plating Cells on Oregon green 488-gelatin Coated Coverslips
  • 05:33Quantification of Fluorescent Gelatin Degradation by Measuring Normalized Matrix Degradation
  • 09:28Results: Invadopodia-mediated ECM Proteolysis
  • 13:23Conclusion

Summary

Automatic Translation

我们描述了典型的方法涂有荧光可视化侵袭伪足介导的基质降解明胶的生产显微镜的盖玻片。利用现有的软件的计算技术,提出了一个混合的人口和量化结果由单个细胞内的基质蛋白水解为多细胞群体,涵盖整个微观领域。

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