JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
定量测量的侵袭伪足介导的细胞外基质蛋白水解单和多细胞的情况下
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
Please note that all translations are automatically generated.
Click here for the English version.
定量测量的侵袭伪足介导的细胞外基质蛋白水解单和多细胞的情况下
DOI:
10.3791/4119-v
•
14:23 min
•
August 27, 2012
•
Karen H. Martin
,
Karen E. Hayes
,
Elyse L. Walk
,
Amanda Gatesman Ammer
,
Steven M. Markwell
,
Scott A. Weed
1
Department of Neurobiology and Anatomy, Program in Cancer Cell Biology, Mary Babb Randolph Cancer Center
,
West Virginia University
Chapters
00:05
Title
01:21
Production of Oregon Green 488-gelatin Coated Coverslips
04:48
Plating Cells on Oregon green 488-gelatin Coated Coverslips
05:33
Quantification of Fluorescent Gelatin Degradation by Measuring Normalized Matrix Degradation
09:28
Results: Invadopodia-mediated ECM Proteolysis
13:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了典型的方法涂有荧光可视化侵袭伪足介导的基质降解明胶的生产显微镜的盖玻片。利用现有的软件的计算技术,提出了一个混合的人口和量化结果由单个细胞内的基质蛋白水解为多细胞群体,涵盖整个微观领域。
Tags
Quantitative Measurement
Invadopodia-mediated
Extracellular Matrix Proteolysis
Single And Multicellular Contexts
Cellular Invasion
Development
Homeostasis
Malregulated Invasion
Cell Movement
Pathological Processes
Inflammation
Cardiovascular Disease
Tumor Cell Metastasis
Proteolytic Degradation
Extracellular Matrix (ECM)
Epithelial Basement Membrane
Endothelial Basement Membrane
Ventral Actin-rich Membrane Protrusive Structures
Invadopodia
Matrix Metalloproteinases (MMPs)
Tumor Cells
In Vitro Analysis
ECM Degradation
Fluorescent Microscopy
Dye-labeled Matrix Proteins
Glass Coverslips
Evaluating Matrix Proteolysis
Cellular Invasive Potential
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在整个细胞群在单细胞水平上研究细胞周期蛋白B的蛋白水解
可视化和分析的mRNA分子,使用荧光<em>在原位</em>杂交<em>酿酒酵母(Saccharomyces cerevisiae)</em
基于探针实时荧光定量PCR途径的小分子RNA定量检测
细胞外离子流的使用离子选择性自引用的微电极技术测量
从富集组织及消化细胞外基质蛋白的成肽质谱分析
高效的哺乳动物细胞表达和细胞外糖蛋白的结晶一步纯化
测量外产酸和分析,以确定糖酵解率
扫描浸软组织的电子显微镜可视化的细胞外基质
一种快速,为隔离,功能可扩展的研究方法,以及细胞衍生的细胞外基质的分析
使用微流体装置来衡量寿命和细胞表型单芽殖酵母细胞
Read Article