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Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
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Chapters
- 00:05Title
- 01:55Designing the Biobrick Synthetic Curli Operon
- 06:06Quantifying and Visualizing Adherent Bacteria on Polystyrene
- 09:01Visualizing Adherent Bacteria on Glass by Microscopy
- 12:22Results: Increased Adherence of Engineered Bacteria
- 14:28Conclusion
The design of a synthetic operon encoding both the secretory apparatus and the structural monomers of curli fibers is described. Overproduction of these amyloids and adherent polymers allows a measurable gain of adherence of the E. coli chassis1. Easy ways to visualize and quantify adherence are explained.
