Journal
/
Bioengineering
/
Engineering adhærerende bakterier ved at skabe en enkelt syntetisk Curli Operon
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering adhærerende bakterier ved at skabe en enkelt syntetisk Curli Operon

DOI:
10.3791/4176-v

•

15:28 min

•

November 16, 2012

•

, , , ,
1UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1,Université de Lyon, 2Département Biosciences, INSA de Lyon,Université de Lyon, 3INSERM U758, Ecole Normale Supérieure de Lyon,Université de Lyon, 4Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon,Université de Lyon

Chapters

  • 00:05Title
  • 01:55Designing the Biobrick Synthetic Curli Operon
  • 06:06Quantifying and Visualizing Adherent Bacteria on Polystyrene
  • 09:01Visualizing Adherent Bacteria on Glass by Microscopy
  • 12:22Results: Increased Adherence of Engineered Bacteria
  • 14:28Conclusion

Summary

Automatic Translation

Automatic Translation

Udformningen af ​​en syntetisk operon kodende både den sekretoriske apparat og de strukturelle monomerer i curli fibre er beskrevet. Overproduktion af disse amyloider og adhærente polymerer giver en målbar gevinst for vedhæftning af den<em> E. coli</em> Chassis<sup> 1</sup>. Nemme måder at visualisere og kvantificere vedhæftning forklares.

Tags

EngineeringAdherent BacteriaSynthetic Curli OperonE. ColiPromoterVisualizationQuantificationBacterial AdherenceSynthetic BiologyBBa K540000 BiobrickTranscriptional SignalsTranslational SignalsCurli RegulationMetal-regulated PromoterQualitative AssessmentQuantitative AssessmentBiofilm Formation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Procedure for Lung Engineering

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Syntetisk, Multi-Layer, Self-Oscillerende Vocal Fold Model Fabrication

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Syntetisk edderkoppespind Produktionen på laboratorieskala

Høj effektivitet, Site-specifik Transfektion af adhærente celler med siRNA Brug mikroelektrode Arrays (MEA)

Densitetsgradient flerlags Polymerisation (DGMP): En ny teknik til at skabe Multi-rum, Customizable stilladser til Tissue Engineering

Optisk påvisning af<em> E. coli</em> bakterier ved Mesoporøse Silicon biosensorer

Nanomanipulation af Single RNA-molekyler af Optisk Pincet

Tissue Engineering af Intrinsic Vaskularisering i en<em> In vivo</em> Tissue Engineering Chamber

Read Article