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Engineering
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紧凑型量子点的单分子成像
JoVE Journal
Engineering
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JoVE Journal
Engineering
Compact Quantum Dots for Single-molecule Imaging
Please note that all translations are automatically generated.
Click here for the English version.
紧凑型量子点的单分子成像
DOI:
10.3791/4236-v
•
17:14 min
•
October 09, 2012
•
Andrew M. Smith
,
Shuming Nie
2
1
Department of Biomedical Engineering
,
Emory University
,
2
Department of Chemistry
,
Georgia Institute of Technology
Chapters
00:05
Title
01:52
Synthesis of Mercury Cadmium Selenide (Hg
x
Cd
1-x
Se) Quantum Dot Cores
06:49
Growth of Cadmium Zinc Sulfide (Cd
y
Zn
1-y
S) Shell
09:20
Phase Transfer
12:01
PEG Coating
13:10
Results: Quantum Dot Characterization
16:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了准备的胶体量子点与最小化的流体力学尺寸为单分子荧光成像。相比传统的量子点,这些纳米颗粒的尺寸类似于球状蛋白质单分子反对光降解,亮度,稳定性和耐非特异性结合的蛋白质和细胞进行了优化。
Tags
Compact Quantum Dots
Single-molecule Imaging
Biomolecular Function
Spatial Heterogeneity
Temporal Heterogeneity
Epifluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Fluorescent Tag
Dye
Protein
Bead
Quantum Dot
High Photon Fluxes
Fluorescence Imaging
Signal Stability
Hydrodynamic Size
Diffusion Behavior
Semiconductor Materials
Photodegradation
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