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Journal
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Immunology and Infection
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通过Bioorthogonal点击化学化学选择性病毒表面改性
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Please note that all translations are automatically generated.
Click here for the English version.
通过Bioorthogonal点击化学化学选择性病毒表面改性
DOI:
10.3791/4246-v
•
12:31 min
•
August 19, 2012
•
Frederick A. Rubino
,
Yoon Hyeun Oum
,
Lakshmi Rajaram
,
Yanjie Chu
,
Isaac S. Carrico
1
Department of Chemistry
,
Stony Brook University
Chapters
00:00
Title
02:04
Production of Aha-Labeled Adenovirus
04:28
Production of Azido-Sugar-Labeled Adenovirus
05:46
Purification of Azide-Labeled Adenoviral Particles
07:34
Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
08:32
Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
09:32
SDS-PAGE Analysis of Clicked Adenovirus
10:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
腺病毒颗粒设计,含有非天然氨基酸类似物azidohomoalanine或叠氮糖<em>Ø</em> GlcNAz。每叠组chemoselectively结扎通过“点击”化学反应病毒表面改性的一种手段。
Tags
Chemoselective Modification
Viral Surfaces
Bioorthogonal Click Chemistry
Gene Therapy
Oncolytic Applications
Vaccine Development
Genetics-based Approaches
Chemoselective Ligation
Targeting Molecules
Dyes
Azide Tags
Metabolic Labeling
Effector Ligands
Adenovirus Particles
Azidohomoalanine
O-linked N-azidoacetylglucosamine
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