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Kemoselektiv Modifikation af virale overflader via Bioorthogonal klik kemi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
DOI:

12:31 min

August 19, 2012

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Chapters

  • 00:00Title
  • 02:04Production of Aha-Labeled Adenovirus
  • 04:28Production of Azido-Sugar-Labeled Adenovirus
  • 05:46Purification of Azide-Labeled Adenoviral Particles
  • 07:34Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
  • 08:32Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
  • 09:32SDS-PAGE Analysis of Clicked Adenovirus
  • 10:34Conclusion

Summary

Automatic Translation

Adenoviruspartikler er manipuleret til at indeholde enten naturlig aminosyre analog azidohomoalanine eller azido sukker<em> O</em>-GlcNAz. Af azidgruppen af ​​hver er kemoselektivt ligeret via "klik"-kemiske reaktioner som et middel til viral overflademodifikation.

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