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Chemoselective Endring av Viral Overflater via Bioorthogonal Klikk kjemi
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Chapters
- 00:00Title
- 02:04Production of Aha-Labeled Adenovirus
- 04:28Production of Azido-Sugar-Labeled Adenovirus
- 05:46Purification of Azide-Labeled Adenoviral Particles
- 07:34Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
- 08:32Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
- 09:32SDS-PAGE Analysis of Clicked Adenovirus
- 10:34Conclusion
Adenovirus partikler er konstruert for å inneholde enten unaturlig aminosyren analoge azidohomoalanine eller azido sukker<em> O</em>-GlcNAz. Den azide gruppe av hver er chemoselectively ligated via "klikk" kjemi reaksjoner som et middel for viral overflate modifikasjon.
Tags
Chemoselective ModificationViral SurfacesBioorthogonal Click ChemistryGene TherapyOncolytic ApplicationsVaccine DevelopmentGenetics-based ApproachesChemoselective LigationTargeting MoleculesDyesAzide TagsMetabolic LabelingEffector LigandsAdenovirus ParticlesAzidohomoalanineO-linked N-azidoacetylglucosamine
