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Høy gjennomstrømning Sekvensiell ELISA for validering av biomarkører for akutt transplantat-mot-vert-sykdom
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
DOI:

09:00 min

October 31, 2012

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Chapters

  • 00:05Title
  • 01:34Experiment Day 0: Sample Preparation and ELISA Test Plate Coating with Capture Antibody for IL-2Ralpha, REG3alpha and HGF
  • 02:48Experiment Day 1: IL-2Ralpha and REG3alpha ELISAs
  • 04:13Experiment Day 1: Elafin and TNFR1 Test Plate Coating with Capture Antibody
  • 04:39Experiment Day 1-2: HGF ELISA
  • 05:46Experiment Day 2: Elafin ELISA
  • 06:16Experiment Day 2: TNFR1 ELISA
  • 06:45Experiment Day 2: IL-8 ELISA
  • 07:25Results: Representative Optical Densities for the Standard Curve of 7 Different ELISA Plates Measuring REG3alpha Concentrations
  • 08:06Conclusion

Summary

Automatic Translation

Høy gjennomstrømning validering av flere kandidater biomarkører kan utføres ved sekvensiell ELISA for å minimere fryse / tine sykluser og bruk av edle plasmaprøver. Her viser vi hvordan du sekvensielt utføre ELISAs for seks ulike validerte plasma biomarkører<sup> 1-3</sup> Av graft-versus-host sykdom (GVHD)<sup> 4</sup> På samme plasmaprøve.

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