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二维和三维活细胞成像的DNA损伤反应蛋白
JoVE Journal
Biology
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JoVE Journal
Biology
Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Please note that all translations are automatically generated.
Click here for the English version.
二维和三维活细胞成像的DNA损伤反应蛋白
DOI:
10.3791/4251-v
•
10:24 min
•
September 28, 2012
•
Jason M. Beckta
2
,
Scott C. Henderson
,
Kristoffer Valerie
2,4
1
Department of Radiation Oncology
,
Virginia Commonwealth University
,
2
Department of Biochemistry & Molecular Biology
,
Virginia Commonwealth University
,
3
Department of Anatomy & Neurobiology
,
Virginia Commonwealth University
,
4
Massey Cancer Center
,
Virginia Commonwealth University
Chapters
00:05
Title
02:07
Cell Preparation
02:41
Microscope Setup and Image Acquisition
07:56
Image Processing and Analysis
09:02
Results: 53BP1 Foci Formation
09:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一个可视化的DNA双链断裂以及其定位在有丝分裂过程中DNA损伤激活的信号蛋白的方法。
Tags
Two-dimensional
Three-dimensional
Live Cell Imaging
DNA Damage Response Proteins
Double-strand Breaks
Mutations
Chromosomal Aberrations
Genomic Instability
DNA Damage Response
Cell Cycle Arrest
Apoptosis
Nonhomologous End-joining
Homologous Recombination Repair
Proteins
53BP1
P53-binding Protein 1
Modified Histones
Foci
Histone Modifications
Chromatin Rearrangement
Article
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