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使用电化学传感器的细菌检测和鉴定
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Bacterial Detection & Identification Using Electrochemical Sensors
DOI:

09:30 min

April 23, 2013

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Chapters

  • 00:05Title
  • 01:39Functionalization of Electrochemical Sensors
  • 03:02Sample Preparation
  • 03:55Electrochemical Sensor Assay
  • 05:14Results: Bacterial Detection & Identification Using Electrochemical Sensors
  • 08:46Conclusion

Summary

Automatic Translation

我们描述了一种电化学传感器的测定方法,细菌的快速检测和识别。的测定法涉及官能化的传感器阵列与DNA的核糖体RNA(rRNA基因)的种属特异性序列的寡核苷酸捕捉探针。目标rRNA基因的捕获探针和辣根过氧化物酶相连的DNA寡核苷酸检测探针的夹心杂交产生可测量的安培电流。

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