A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Research Service,Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine,University of California, Los Angeles , 3GeneFluidics, 4Division of Infectious Diseases,Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics,University of California, Los Angeles
Chapters
- 00:05Title
- 01:39Functionalization of Electrochemical Sensors
- 03:02Sample Preparation
- 03:55Electrochemical Sensor Assay
- 05:14Results: Bacterial Detection & Identification Using Electrochemical Sensors
- 08:46Conclusion
Vi beskriver en elektrokjemisk sensor assay metode for deteksjon og rask bakteriell identifikasjon. Analysen innebærer en sensor array funksjonaliseres med DNA oligonukleotide fangst prober for ribosomale RNA (rRNA) artsspesifikke sekvenser. Sandwich hybridisering av target rRNA med fangst sonde og en pepperrotperoksidase-linked DNA oligonukleotid detektor probe produserer en målbar amperometrisk strøm.
Tags
Bacterial DetectionIdentificationElectrochemical SensorsBlood Glucose MeasurementAnalyte DetectionSignal TransductionRedox EnzymeAmperometric ElectrodeSensor SpecificityGlucose SensorDNA OligonucleotidesSandwich HybridizationRibosomal RNA (rRNA)Capture ProbeDetector ProbeHorseradish Peroxidase (HRP)Substrate OxidationCurrent Flow
