A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:56Construction of a Fluorescent Bacterial Strain via Conjugation
- 02:22Production of Axenic Nematode Eggs
- 04:45Co-cultivation Assay with Fluorescent Bacteria
- 05:52Collection and Screening of Nematodes for Bacterial Association by Microscopy
- 06:42Results: Representative Nematode and Bacteria Imagery
- 08:33Conclusion
Om de mutualisme tussen studeren<em> Xenorhabdus</em> Bacteriën en<em> Steinernema</em> Nematoden, methoden ontwikkeld om bacteriële aanwezigheid en locatie binnen nematoden volgen. De experimentele benadering, die kan worden toegepast op andere systemen, houdt techniek bacteriën het groen fluorescent eiwit en visualiseren expressie, met fluorescentiemicroscopie bacteriën in de transparante nematode.
Tags
VisualizingBacteriaNematodesFluorescent MicroscopySymbiotic AssociationsXenorhabdus BacteriaSteinernema NematodesModel SystemEntomopathogenicBacterial SymbiontIntestineInfective Juvenile StageNematode SpeciesInteractionsVisualization MethodOptical TransparencyMicroscopyFluorescent ProteinPlasmidsEscherichia Coli Strain
