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Chapters
- 00:05Title
- 01:25Embryoid Body Formation
- 03:04Neuroectoderm Induction and Neuron Formation
- 04:25Representative Neuron Generation Results
- 07:06Conclusion
Protokoller for neuronal differensiering av pluripotent menneskelige stamceller (hPSCs) er ofte tidkrevende og krever betydelige cellekultur ferdigheter. Her har vi tilpasset et lite molekyl-basert differensiering prosedyren til en multititre plate format, slik at enkel, rask, og effektiv produksjon av humane nevroner på en kontrollert måte.
Tags
NeuronsHuman Pluripotent Stem CellsMultititre Plate FormatProtocolsGenerationNeuroectodermDifferentiationSignaling PathwaysTGFβ/SMAD2BMP/SMAD1FGF/ERKFunctional NeuronsReproducibilityMid-throughput ApplicationsFloating SpheresAdherent ConditionsBipolar Sensory NeuronsEfficient ProcedureChemically Defined MediumSmall MoleculesFunctional InvestigationCell-based Screening Approaches
