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Chapters
- 00:05Title
- 01:30Choose the Brightest Embryo and Embedding It for Photoconversion Assay
- 02:52Photoconversion and Embryonic Development and Tracking Photoconverted Tissue
- 05:11Representative Photoconversion Results
- 06:56Conclusion
Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.
