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激光伤害的斑马鱼胚胎骨骼肌
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Biology
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JoVE Journal
Biology
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
Please note that all translations are automatically generated.
Click here for the English version.
激光伤害的斑马鱼胚胎骨骼肌
DOI:
10.3791/4351-v
•
07:13 min
•
January 30, 2013
•
Cécile Otten
,
Salim Abdelilah-Seyfried
1
Max Delbrück Center for Molecular Medicine
Chapters
00:05
Title
01:20
Labeling of Single Cells
02:58
Embryo Laser Wounding, Recovery, and Analysis
04:29
Representative Muscle Wounding Results
06:40
Conclusion
Summary
Automatic Translation
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这里介绍的方法,包括精确的具有高能量的激光脉冲和随后的分析随时间的伤害和其恢复损伤的活斑马鱼胚胎。我们还展示了如何转基因标记的单或骨骼肌细胞可以被跟踪和激光损伤后。
Tags
Zebrafish
Embryonic Skeletal Muscle
Laser-inflicted Injury
Muscle Regeneration
Myotoxin Injections
Muscle Transplantations
Denervation-devascularization Induced Regeneration
Intensive Exercise
Murine Muscular Dystrophy Models
Mdx Mouse
Runzel2
Sapje3
Antisense Oligonucleotide Morpholinos
Dystrophy-associated Genes
Galanthamine
Acetylcholinesterase Inhibitor
Hypercontraction
Laser-mediated Cell Ablation
Two-photon Laser
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