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高效率，站点特定的贴壁细胞转染siRNA的微电极阵列（MEA）
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Bioengineering
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JoVE Journal Bioengineering
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
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高效率，站点特定的贴壁细胞转染siRNA的微电极阵列（MEA）

DOI:
10.3791/4415-v

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09:14 min

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September 13, 2012

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,
1School of Biological and Health Systems Engineering,Arizona State University

Chapters

  • 00:05Title
  • 01:26MEA Preparation and Cell Seeding
  • 03:58Site-specific Transfection of siRNA and Electroporation
  • 08:00Results: High Efficiency, Site-specific Transfection of Adherent Cells with siRNA using MEA
  • 08:52Conclusion

Summary

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文章详细介绍了协议的站点特定的扰码序列的siRNA转染贴壁哺乳动物细胞培养用微电极阵列（MEA）。

Tags

SiRNAShRNARNAi PathwayFunctional GenomicsTherapeuticsDelivery TechniquesElectroporationChemical Transfection MethodsCationic LipidsCationic PolymersNanoparticlesHigh-throughput TechnologiesSingle Cell StudiesCell-cell InteractionsGene DeliveryMicroscale Electroporation

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