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肽:MHC四聚体为基础的抗原表位特异性的T细胞的富集
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
DOI:

13:58 min

October 22, 2012

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Chapters

  • 00:05Title
  • 01:50Cell Isolation from Lymphoid Tissue
  • 03:35Tetramer Staining
  • 04:18Magnetic Enrichment
  • 06:39Flow Cytometry
  • 09:07Results: Calculation of MHC Tetramer-based Enrichment of Epitope-specific T Cells
  • 13:15Conclusion

Summary

Automatic Translation

此协议描述了使用的肽:MHC四聚体和磁性微珠分离低频表位特异性的T细胞群体,并通过流式细胞术分析它们。这种方法能够直接研究内源性T细胞群的兴趣<em在体内</em实验系统。

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