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Immunology and Infection
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肽:MHC四聚体为基础的抗原表位特异性的T细胞的富集
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JoVE Journal
Immunology and Infection
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Please note that all translations are automatically generated.
Click here for the English version.
肽:MHC四聚体为基础的抗原表位特异性的T细胞的富集
DOI:
10.3791/4420-v
•
13:58 min
•
October 22, 2012
•
Francois P. Legoux
,
James J. Moon
1
Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit
,
Massachusetts General Hospital and Harvard Medical School
Chapters
00:05
Title
01:50
Cell Isolation from Lymphoid Tissue
03:35
Tetramer Staining
04:18
Magnetic Enrichment
06:39
Flow Cytometry
09:07
Results: Calculation of MHC Tetramer-based Enrichment of Epitope-specific T Cells
13:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此协议描述了使用的肽:MHC四聚体和磁性微珠分离低频表位特异性的T细胞群体,并通过流式细胞术分析它们。这种方法能够直接研究内源性T细胞群的兴趣<em在体内</em实验系统。
Tags
Peptide:MHC Tetramer-based Enrichment
Epitope-specific T Cells
T Cell Antigen Receptor Specificity
Functional Response
Proliferation
Cytokine Production
Activation Markers
TCR Transgenic Adoptive Transfer Model
Monoclonal T Cells
Histocompatible Hosts
Congenic Marker Antibody
Experimental Artifacts
Functional Heterogeneity
Polyclonal Population
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