JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
研究DNA损伤检验点<em>爪蟾</em>卵提取物
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Study of the DNA Damage Checkpoint using
Xenopus
Egg Extracts
Please note that all translations are automatically generated.
Click here for the English version.
研究DNA损伤检验点<em>爪蟾</em>卵提取物
DOI:
10.3791/4449-v
•
10:55 min
•
November 05, 2012
•
Jeremy Willis*
1
,
Darla DeStephanis*
1
,
Yogin Patel
,
Vrushab Gowda
,
Shan Yan
1
Department of Biology
,
University of North Carolina at Charlotte
Chapters
00:05
Title
01:44
Low Speed Extract Preparation
05:46
Preparation of a DNA Damage-mimicking Structure (AT70)
06:31
DNA Damage: MMS-treated Sperm Chromatin
07:35
DNA Damage: UV-treated Sperm Chromatin
07:58
Triggering the DNA Damage Checkpoint in LSE
08:54
Induce DNA Damage Checkpoint in LSE with the AT70
09:35
Results: DNA Damage Checkpoint of Xenopus Egg Extracts
10:05
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
非洲爪蟾
卵提取物是一个有用的模型系统,研究DNA损伤检验点。该协议是
非洲爪蟾
卵提取物和DNA损伤检验点的诱导试剂的准备。这些技术是适应各种DNA损伤的方法,在研究的DNA损伤检查点的信号。
Tags
DNA Damage Checkpoint
Xenopus Egg Extracts
Endogenous Insults
Environmental Insults
DNA Damage Checkpoint Signaling
Sensors
Transducers
Effectors
ATR (ATM And Rad3-related)
Kinases
Chk1 (Checkpoint Kinase 1)
Cell Cycle Arrest
Transcription Activation
DNA Damage Repair
Apoptosis
Senescence
Genomic Instability
Genomic Integrity
Human Diseases
Cancer Development
Xenopus Laevis Egg Extracts
Cell-free Extract Model System
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测试的速度和运动方向的视觉灵敏度在蜥蜴
计算机生成的动物模型的刺激
制备及分馏爪蟾卵提取
在使用分馏爪蟾卵提取物的体外核大会
施肥
爪蟾卵母细胞
使用主机传输方法
二维和三维活细胞成像的DNA损伤反应蛋白
生产<em>热带爪蟾</em>卵提取物识别微管相关的RNA
重组β-catenin的降解<em>爪蟾</em>卵提取物
单工程的RNA转录物的活细胞实时成像采用比例式双分子信标
检测和DNA损伤分析小鼠骨骼肌<em>原位</em>使用TUNEL法
Read Article