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Chapters
- 00:05Title
- 01:44Low Speed Extract Preparation
- 05:46Preparation of a DNA Damage-mimicking Structure (AT70)
- 06:31DNA Damage: MMS-treated Sperm Chromatin
- 07:35DNA Damage: UV-treated Sperm Chromatin
- 07:58Triggering the DNA Damage Checkpoint in LSE
- 08:54Induce DNA Damage Checkpoint in LSE with the AT70
- 09:35Results: DNA Damage Checkpoint of Xenopus Egg Extracts
- 10:05Conclusion
Xenopus yumurta özü DNA hasarı kontrol noktasında araştırmak için yararlı bir model sistemidir. Bu protokol, Xenopus yumurta özü ve DNA hasarına neden olan kontrol noktası reaktiflerin hazırlanması içindir. Bu teknikler DNA hasar kontrol noktası sinyalleşmenin çalışmada DNA hasarına yaklaşımların çeşitli uyarlanabilir.
Tags
DNA Damage CheckpointXenopus Egg ExtractsEndogenous InsultsEnvironmental InsultsDNA Damage Checkpoint SignalingSensorsTransducersEffectorsATR (ATM And Rad3-related)KinasesChk1 (Checkpoint Kinase 1)Cell Cycle ArrestTranscription ActivationDNA Damage RepairApoptosisSenescenceGenomic InstabilityGenomic IntegrityHuman DiseasesCancer DevelopmentXenopus Laevis Egg ExtractsCell-free Extract Model System
