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Blastomer Eksplantater at teste for Cell Fate Commitment under fosterudviklingen
JoVE Journal
Biology
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JoVE Journal Biology
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
DOI:

14:08 min

January 26, 2013

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Chapters

  • 00:05Title
  • 02:02Preparation of Instruments, Culture Media and Dishes
  • 04:19Selection of Embryos
  • 06:36Preparation of Explants
  • 10:01Harvesting Explants for Analysis
  • 10:39Analysis of Gene and Protein Expression: Representative Results
  • 13:03Conclusion

Summary

Automatic Translation

Den skæbne af en individuel embryocelle kan påvirkes af arvelige molekyler og / eller ved hjælp af signaler fra naboceller. Anvendelse skæbne kort over spaltning fase Xenopus embryo, kan enkelte blastomeres identificeres til dyrkning isoleret for at vurdere bidraget af nedarvede molekyler versus celle-celle interaktioner.

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