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Sequencial de foto-branqueamento de delinear células de Schwann individuais na junção neuromuscular
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1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München
Chapters
- 00:05Title
- 01:36Preparing the Triangularis Sterni Explant
- 04:34Photobleaching and Time-lapse Microscopy
- 07:52Fixing and Dissecting the Explant for Immunohistochemistry
- 09:45Triangularis Sterni Visualization with GFP in Schwann Cells
- 10:42Conclusion
Visualizando as células individuais em tecidos densamente compactados, tais como as células de Schwann terminais (SCs) em junções neuromusculares (NMJs), é um desafio. "Sequential foto-branqueamento" permite delinear SCs terminais individuais, por exemplo, no explante triangularis músculo Sterni, uma preparação conveniente nervo-músculo, onde sequencial de branqueamento podem ser combinados com o lapso de tempo de imagem e<em> Post-hoc</em> Imunomarcações.
Tags
Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling
