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Hele Mount RNA TL<em> In situ</em> Hybridisatie van<em> Drosophila</em> Embryo's

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Biology

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JoVE Journal Biology

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

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Hele Mount RNA TL<em> In situ</em> Hybridisatie van<em> Drosophila</em> Embryo's

DOI:

10.3791/50057-v

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09:57 min

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January 30, 2013

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Félix Legendre*^1,2, Neal Cody*¹, Carole Iampietro, Julie Bergalet, Fabio Alexis Lefebvre², Gaël Moquin-Beaudry², Olivia Zhang, Xiaofeng Wang, Eric Lécuyer²

¹Institut de Recherches Cliniques de Montréal (IRCM), ²Department of Biochemistry,Université de Montréal

Chapters

00:05Title
02:15Collection and Fixation of Drosophila Embryos
04:44Post-fixation Processing
05:58Hybridization and Post-hybridization Washes
08:14FISH Showing Subcellular Localization of mRNA in Drosophila Embryos
09:06Conclusion

Summary

Automatic Translation

Hier beschrijven we een geheel gemonteerde fluorescent<em> In situ</em> Hybridisatie (FISH)-protocol voor het bepalen van de expressie en lokalisatie eigenschappen van RNA's die tijdens de embryogenese in de fruitvlieg,<em> Drosophila melanogaster</em>.

Tags

RNA In Situ Hybridization RNA-ISH Fluorescent In Situ Hybridization FISH Tyramide Signal Amplification TSA Gene Expression RNA Localization Drosophila Embryos Developmental Biology

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