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Hel Mount RNA Lysrör<em> In situ</em> Hybridisering av<em> Drosophila</em> Embryon
JoVE Journal
Biology
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JoVE Journal Biology
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
DOI:

09:57 min

January 30, 2013

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Chapters

  • 00:05Title
  • 02:15Collection and Fixation of Drosophila Embryos
  • 04:44Post-fixation Processing
  • 05:58Hybridization and Post-hybridization Washes
  • 08:14FISH Showing Subcellular Localization of mRNA in Drosophila Embryos
  • 09:06Conclusion

Summary

Automatic Translation

Här beskriver vi en hel-mount lysrör<em> In situ</em> Hybridisering (FISH) protokoll för att bestämma uttrycket och lokalisering egenskaper RNA uttrycks under embryogenes i bananfluga,<em> Drosophila melanogaster</em>.

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