Journal
/
Neuroscience
/
实验方法测试的影响，在怀孕期间对酒精诱导的细胞凋亡在C57BL / 6小鼠神经营养肽，ADNF-9
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice
Please note that all translations are automatically generated. Click here for the English version.

实验方法测试的影响，在怀孕期间对酒精诱导的细胞凋亡在C57BL / 6小鼠神经营养肽，ADNF-9

DOI:
10.3791/50092-v

•

09:50 min

•

April 24, 2013

•

1Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences,University of Toledo

Chapters

  • 00:05Title
  • 01:26Alcohol Dosing and Trophic Peptide Treatment
  • 02:42Dissection of Fetuses and Microdissection of Fetal Brains
  • 04:32Embedding Fetal Brains in Gelatin for Sectioning
  • 06:07TUNEL Reaction for Detection of Apoptosis
  • 08:16Results: Effects of the Neurotrophic Peptide, ADNF-9 Against Alcohol-induced Apoptosis
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

这里重点提出的实验设计，研究酒精暴露在怀孕期间胎儿大脑细胞凋亡和应用营养神经肽的影响。从养殖到胎儿大脑的集合的详细描述。细胞凋亡的测定技术进行详细说明。

Tags

Experimental MethodsNeurotrophic PeptideADNF-9Alcohol-induced ApoptosisPregnancyC57BL/6 MiceFetal Brain GrowthMicrodissectionTUNEL AssayApoptosisCell DeathFetal Alcohol ExposureLiquid DietBreedingVibratome Sectioning

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在哺乳动物的中枢神经系统视神经横断后的实验操作方法

器官型小脑文化：细胞凋亡的挑战和检测

测量小鼠运动协调

Cortex-，Hippocampus-，Thalamus-，下丘脑 - ，横向隔核 - 纹状体专用<em>在子宫内</em>电穿孔的C57BL / 6小鼠

实验模型研究酸性后处理对脑缺血的神经保护作用

MOG35-55 诱发自身免疫性脑脊髓炎 C57BL/6 小鼠矢状面运动步态分析

C57BL/6 小鼠自身免疫性神经炎的功能和病理评价的简易方法

c57bl6 小鼠耳蜗植入术与电唤起脑干反应记录

小鼠体内合成蛋白的定量测量

Social Defeat Stress Model for Adolescent C57BL/6 Male and Female Mice

Read Article