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Bioengineering
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出芽酵母の老化複製の連続高分解能顕微鏡観察
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
Please note that all translations are automatically generated.
Click here for the English version.
出芽酵母の老化複製の連続高分解能顕微鏡観察
DOI:
10.3791/50143-v
•
10:41 min
•
August 20, 2013
•
Daphne H. E. W. Huberts
,
Georges E. Janssens
,
Sung Sik Lee
,
Ima Avalos Vizcarra
,
Matthias Heinemann
5
1
Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute
,
University of Groningen
,
2
Department for the Biology of Ageing, European Research Institute for the Biology of Ageing
,
University Medical Centre Groningen, University of Groningen
,
3
Institute of Biochemistry
,
ETH Zurich
,
4
Department of Health Sciences and Technology
,
ETH Zurich
,
5
Institute of Molecular Systems Biology
,
ETH Zurich
Chapters
00:05
Title
01:32
Production and Preparation of a Silicon Wafer Mold
02:14
Production of Microfluidic Chips
05:10
Preparing the Chip for Cell Loading
06:20
Loading of Yeast Cells into the Microfluidic Chip
07:53
Results: Observation of Replicative Aging of Budding Yeast
10:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここではそれらの完全な複製および/または時系列の寿命の間に単一の出芽酵母細胞の連続的かつ高分解能顕微鏡イメージングを可能にするマイクロ流体装置の動作を説明する。
Tags
Microfluidic Chip
Single Cell Tracking
Budding Yeast
Replicative Aging
Cell Retention
High-resolution Microscopy
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