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Isolering og immunfarvning Lymfocytter og dendritiske celler fra murine Peyerske plaks
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
DOI:

09:49 min

March 17, 2013

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Chapters

  • 00:05Title
  • 01:40Isolation of PP Cells for Flow Cytometry
  • 03:03Antibody Labeling of Cells for Flow Cytometry
  • 03:48Preparation of PP Cryosections
  • 04:49Cryosectioning
  • 05:20Antibody Labeling of Cryosections and Confocal Microscopy Analysis
  • 06:11Results: Immunological Analyses of Mouse Peyer’s Patches
  • 09:23Conclusion

Summary

Automatic Translation

Der er en stigende interesse for at forstå de immunologiske funktioner af specifikke subpopulationer af celler i Peyerske plaques (PP), de primære induktive steder af tarm-associerede lymfoide væv. Her skitserer vi parallelle protokoller til fremstilling PP encellede forberedelserne til flowcytometrisk analyse og PP kryosektioner for immunfarvning.

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