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JoVE Journal
Immunology and Infection

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Isolering og immunfarvning Lymfocytter og dendritiske celler fra murine Peyerske plaks
 
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Isolering og immunfarvning Lymfocytter og dendritiske celler fra murine Peyerske plaks

Article DOI: 10.3791/50167
March 17th, 2013

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Summary March 17th, 2013

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Der er en stigende interesse for at forstå de immunologiske funktioner af specifikke subpopulationer af celler i Peyerske plaques (PP), de primære induktive steder af tarm-associerede lymfoide væv. Her skitserer vi parallelle protokoller til fremstilling PP encellede forberedelserne til flowcytometrisk analyse og PP kryosektioner for immunfarvning.

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