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Isolering og immunfarvning Lymfocytter og dendritiske celler fra murine Peyerske plaks
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Chapters
- 00:05Title
- 01:40Isolation of PP Cells for Flow Cytometry
- 03:03Antibody Labeling of Cells for Flow Cytometry
- 03:48Preparation of PP Cryosections
- 04:49Cryosectioning
- 05:20Antibody Labeling of Cryosections and Confocal Microscopy Analysis
- 06:11Results: Immunological Analyses of Mouse Peyer’s Patches
- 09:23Conclusion
Der er en stigende interesse for at forstå de immunologiske funktioner af specifikke subpopulationer af celler i Peyerske plaques (PP), de primære induktive steder af tarm-associerede lymfoide væv. Her skitserer vi parallelle protokoller til fremstilling PP encellede forberedelserne til flowcytometrisk analyse og PP kryosektioner for immunfarvning.
