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Journal
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Immunology and Infection
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隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑
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Immunology and Infection
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Immunology and Infection
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
Please note that all translations are automatically generated.
Click here for the English version.
隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑
DOI:
10.3791/50167-v
•
09:49 min
•
March 17, 2013
•
Magdia De Jesus
,
Sarita Ahlawat
,
Nicholas J. Mantis
1
Division of Infectious Diseases
,
New York State Department of Health
Chapters
00:05
Title
01:40
Isolation of PP Cells for Flow Cytometry
03:03
Antibody Labeling of Cells for Flow Cytometry
03:48
Preparation of PP Cryosections
04:49
Cryosectioning
05:20
Antibody Labeling of Cryosections and Confocal Microscopy Analysis
06:11
Results: Immunological Analyses of Mouse Peyer’s Patches
09:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
理解在派伊尔氏淋巴集结的特定的细胞亚群(PPS),初级感应网站肠相关的淋巴组织的免疫功能有越来越大的兴趣。在这里,我们列出了流式细胞仪分析和PP冰冻切片免疫准备的PP单细胞准备的并行协议。
Tags
Peyer’s Patches
Intestinal Immunosurveillance
M Cells
Follicle-associated Epithelium (FAE)
Dendritic Cells (DCs)
Macrophages
Lymphocytes
Flow Cytometry
Cryosectioning
Immunostaining
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