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采用实时定量PCR检测供体细胞植入一个具有竞争力的小鼠骨髓移植模型
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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
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采用实时定量PCR检测供体细胞植入一个具有竞争力的小鼠骨髓移植模型

DOI:
10.3791/50193-v

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10:11 min

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March 07, 2013

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1Division of Hematology-oncology, Department of Medicine,Medical University of South Carolina

Chapters

  • 00:05Title
  • 01:42Bone Marrow Cell Isolation
  • 04:34Competitive Bone Marrow Transplantation, Sample Collection, and Genomic DNA Isolation
  • 06:25Standard Curve Preparation and Real-time PCR
  • 07:47Representative Donor Cell Engraftment Results
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

确定供体细胞移植在小鼠骨髓移植模型中，缺乏定义的表型标记提出了挑战。我们描述了一种方法来量化男性捐赠者的细胞植入女性移植受体小鼠。此方法可用于研究HSC功能在所有的小鼠品系。

Tags

Quantitative Real-time PCRDonor Cell EngraftmentCompetitive Murine Bone Marrow TransplantationHematopoietic Stem Cell FunctionZfy1Bcl2Flow Cytometry

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