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Chapters
- 00:05Title
- 01:42Bone Marrow Cell Isolation
- 04:34Competitive Bone Marrow Transplantation, Sample Collection, and Genomic DNA Isolation
- 06:25Standard Curve Preparation and Real-time PCR
- 07:47Representative Donor Cell Engraftment Results
- 09:46Conclusion
Bestemmelse donorcelle engraftment er en udfordring i mus knoglemarvstransplanterede modeller, der mangler veldefinerede fænotypiske markører. Vi beskrev en metode til at kvantificere mandlig donorcelle indpodning hos kvindelige transplantation recipientmus. Denne metode kan anvendes i alle musestammer til studiet af HSC funktioner.
