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使用反时相蛋白的阵列(RPPA)探索在个人的肾细胞癌蛋白表达的变化
JoVE Journal
Medicine
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JoVE Journal Medicine
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
DOI:

12:22 min

January 22, 2013

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Chapters

  • 00:05Title
  • 01:39Identification of Morphological and Molecular Tumor Heterogeneity
  • 02:58Protein Extraction from Tumor Samples
  • 04:24RPPA Printing
  • 06:00RPPA Protein Detection
  • 09:27Results: Pre- and Post-TKI-treated Renal Cell Cancer Show Differential Protein Expression
  • 12:06Conclusion

Summary

Automatic Translation

RPPA支持数百个样品,印在硝酸纤维素幻灯片同时进行审讯,用荧光标记的抗体蛋白的表达。这项技术已被应用在肾透明细胞癌的药物治疗的非均质性研究的效果。

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