Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
01:20Intravital Microscope and Cremaster Muscle Preparation for Intravital Microscropy
02:30Intravital Microscopy for TNFα-induced Vascular Inflammation
03:33Data Analysis for the Venular Inflammation Model
06:06Intravital Microscopy for Laser-induced Arteriolar Thrombosis
06:58Data Analysis for the Arteriolar Thrombosis Model
08:20Results: Representative Heterotypic Interactions of Platelets and Neutrophils during TNFα-induced Vascular Inflammation and Platelet Thrombus at the Site of Laser-induced Arteriolar Injury in Live Mice
Her rapporterer vi en eksperimentell teknikk av fluorescens intravital mikroskop for å visualisere heterotypic blodplatelignende nøytrofile interaksjoner på aktivert endotel under vaskulær inflammasjon og trombedannelse i levende mus. Denne mikroskopiske teknologien vil være verdifullt å studere molekylære mekanismen for vaskulær sykdom og å teste farmakologiske stoffer i henhold patofysiologiske forhold.