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En Multiplexed Luciferase-basert screening plattform for avhør kreft-assosiert Signal Transduksjon i dyrkede celler
JoVE Journal
Medicine
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JoVE Journal Medicine
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
DOI:

10:13 min

July 03, 2013

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Chapters

  • 00:05Title
  • 01:54Preparation of Cells for siRNA and Reporter Transfection
  • 03:19siRNA and Reporter Transfection of Cells
  • 03:22Preparing Reporter Construct DNA
  • 04:10Preparing siRNA/DNA Transfection Mixtures
  • 05:42Adding Transfection Mixtures to Cells
  • 06:21Determine Luciferase Activities from Cell Samples
  • 08:04Results: High-throughput Interrogation of Gene Function in Wnt/β-catenin, p53, and Kras Singaling
  • 09:53Conclusion

Summary

Automatic Translation

Oppnå et system nivå forståelse av cellulære prosesser er et mål for dagens cellebiologi. Vi beskriver her strategier for multipleksing luciferase reportere i ulike cellular funksjon endepunkter å avhøre gen-funksjon ved hjelp av genom-skala RNAi biblioteker.

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