JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
多路复用讯问癌症相关的信号转导在培养的细胞中的荧光素酶的筛选平台
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
多路复用讯问癌症相关的信号转导在培养的细胞中的荧光素酶的筛选平台
DOI:
10.3791/50369-v
•
10:13 min
•
July 03, 2013
•
Ozlem Kulak
,
Lawrence Lum
1
Department of Cell Biology
,
UT Southwestern Medical Center
Chapters
00:05
Title
01:54
Preparation of Cells for siRNA and Reporter Transfection
03:19
siRNA and Reporter Transfection of Cells
03:22
Preparing Reporter Construct DNA
04:10
Preparing siRNA/DNA Transfection Mixtures
05:42
Adding Transfection Mixtures to Cells
06:21
Determine Luciferase Activities from Cell Samples
08:04
Results: High-throughput Interrogation of Gene Function in Wnt/β-catenin, p53, and Kras Singaling
09:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
实现系统级的细胞过程的理解,是现代细胞生物学的目标。我们在这里描述的各种细胞功能的复用荧光素酶记者的战略点审问基因的功能基因组规模的RNAi库。
Tags
Multiplexed Reporter System
RNA Interference
RNAi
Genome-scale Gene Function
Cancer Cell Vulnerabilities
High-content Screening
Molecular Targeted Therapy
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
如何从MR图像测量皮质折叠:一个循序渐进的教程,以计算本地Gyrification指数
在神经退行性疾病的高内涵筛选
的的膀胱测压小老鼠:A膀胱Chemosensation研究的使用
对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选
自愿屏气技术减少心脏剂量的左乳房放射治疗
颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析
研究胰腺癌干细胞特性开发新的治疗策略
高含量<em>体外</em>胰岛β细胞复制发现平台
筛选生物活性纳米颗粒在噬菌体免疫细胞中的Toll样受体信号传导抑制剂
血小板输血中微粒的常规筛选方法
Read Article