Journal
/
Biology
/
对小鼠胰腺胰岛分离和细胞内cAMP的测定方法
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Please note that all translations are automatically generated. Click here for the English version.

对小鼠胰腺胰岛分离和细胞内cAMP的测定方法

DOI:
10.3791/50374-v

•

12:33 min

•

June 25, 2014

•

, , ,
1Department of Nutrional Sciences,University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism,University of Wisconsin-Madison, 3School of Pharmacy,University of Waterloo

Chapters

  • 00:05Title
  • 01:13Inflating the Pancreas
  • 03:47Pancreas Removal
  • 04:57Washing
  • 08:17Picking Islets
  • 09:16cAMP Assay
  • 11:19Results: Secreted Insulin can be Measured from the cAMP Stimulation Medium
  • 12:09Conclusion

Summary

Automatic Translation

Automatic Translation

含量测定采用胰岛分离的小鼠胰岛体外 β细胞功能是在糖尿病病理生理学和治疗学研究的一个重要组成部分。虽然许多下游应用的情况下，该协议特别描述作为必要参数确定的β-细胞功能的细胞内环磷酸腺苷（cAMP）的测定。

Tags

Pancreatic Islet IsolationIntracellular CAMP DeterminationDiabetes PathophysiologyBeta-cell FunctionIncretin ReceptorsType 1 DiabetesType 2 Diabetes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

小鼠胰岛隔离

人类胰岛的分离：第一部分：收集和胰腺组织消化

人类胰岛的分离：第二部分：人类胰岛的分离纯化及文化

计算机辅助大型胰岛质量，粒度分布和结构的可视化和量化

小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用

小鼠原代胰腺腺泡细胞的分离和培养

近红外（NIR）光增加线粒体功能的标志物的表达，在鼠标前庭上皮细胞

核酸的分离过滤：一种简单快速的DNA提取方法

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

胰腺胰岛与小鼠分离的简单高效协议

Read Article