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JoVE Journal Chemistry
Ongerichte Metabolomics uit biologische grondstoffen gebruiken UltraPerformance Liquid Chromatography-hoge resolutie massaspectrometrie (UPLC-HRMS)
An Externally-Heated Diamond Anvil Cell for Synthesis and Single-Crystal Elasticity Determination of Ice-VII at High Pressure-Temperature Conditions
Journal (Chemistry)
An Externally-Heated Diamond Anvil Cell for Synthesis and Single-Crystal Elasticity Determination of Ice-VII at High Pressure-Temperature Conditions
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
Journal (Chemistry)
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
 
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Ongerichte Metabolomics uit biologische grondstoffen gebruiken UltraPerformance Liquid Chromatography-hoge resolutie massaspectrometrie (UPLC-HRMS)

Article DOI: 10.3791/50433
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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May 20th, 2013

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Ongerichte metabolomics biedt een hypothese genererende momentopname van een metabool profiel. Dit protocol zal de extractie en analyse van metabolieten uit cellen, serum of weefsel tonen. Een reeks van metabolieten worden onderzocht met behulp van vloeistof-vloeistof fase-extractie, microflow UltraPerformance vloeistofchromatografie / hoge-resolutie massaspectrometrie (UPLC-HRMS) gekoppeld aan differentiële analyse software.

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