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JoVE Journal Chemistry
Metabolómica no focalizados de fuentes biológicas Uso UltraPerformance cromatografía líquida-espectrometría de masas de alta resolución (UPLC-HRMS)
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
Journal (Chemistry)
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
Journal (Chemistry)
Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
 
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Metabolómica no focalizados de fuentes biológicas Uso UltraPerformance cromatografía líquida-espectrometría de masas de alta resolución (UPLC-HRMS)

Article DOI: 10.3791/50433
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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May 20th, 2013

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Untargeted metabolómica ofrece una hipótesis de generación instantánea de un perfil metabólico. Este protocolo se demostrará la extracción y el análisis de los metabolitos de las células, suero, o tejidos. Una gama de metabolitos se examinó utilizando extracción en fase líquido-líquido, microflujo UltraPerformance cromatografía líquida / de alta resolución de la espectrometría de masas (UPLC-HRMS) acoplado al software de análisis diferencial.

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