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Biology
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MBP基于标记策略的Mgm101重组蛋白的制备
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
Please note that all translations are automatically generated.
Click here for the English version.
MBP基于标记策略的Mgm101重组蛋白的制备
DOI:
10.3791/50448-v
•
11:40 min
•
June 25, 2013
•
Xiaowen Wang
,
MacMillan Mbantenkhu
,
Sara Wierzbicki
,
Xin Jie Chen
1
Department of Biochemistry and Molecular Biology
,
State University of New York Upstate Medical University
Chapters
00:05
Title
02:10
Construction of MBP-Mgm101 Fusion Protein
03:16
Expression, Induction, Cell Lysis and DNase I Treatment
05:28
Purification on Amylose Column
07:07
PreScission Protease Cleavage and Cation Exchange Chromatography
08:42
Size Exclusion Chromatography
10:27
Analysis of Purified Mgm101
11:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酵母线粒体类核蛋白质,Mgm101,是一种重组蛋白RAD52型,形成大低聚环。甲协议的描述以制备可溶性的重组Mgm101使用麦芽糖结合蛋白(MBP)标记加上阳离子交换和尺寸排阻色谱法策略。
Tags
Mgm101
Recombinant Protein
MBP-tagged
E. Coli Expression
Affinity Chromatography
Cationic Exchange Chromatography
Size Exclusion Chromatography
Mitochondrial DNA Repair
Rad52-type Recombination Proteins
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