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长时程增强一个非常稳定的和可重复的记录和保存海马鼠标片制备工艺的改进

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

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长时程增强一个非常稳定的和可重复的记录和保存海马鼠标片制备工艺的改进

DOI:

10.3791/50483-v

•

09:39 min

•

June 26, 2013

•

Agnès Villers, Laurence Ris

¹Department of Neurosciences, Research Institute for Biosciences,University of Mons

Chapters

00:05Title
01:29Preparation of the Electrophysiological Rig and the Brain Slice Recording Chamber
02:27Brain Removal and Hippocampal Dissection
04:51Hippocampal Slices Preparation
05:56Incubation of Slices in the Chamber
07:02Results: Induction of LTP in Hippocampal Slices
09:04Conclusion

Summary

Automatic Translation

本文提出了一种完整的方法来准备和保存<em>在体外</em>急性成年小鼠海马脑片。该协议允许非常稳定持久的长时程增强（LTP）的记录超过8小时，95％的成功率。

Tags

Hippocampal Slices Long-term Potentiation LTP Synaptic Plasticity Memory Encoding CA1 Region Experimental Conditions Oxygenation Temperature Humidity Slice Orientation Slice Viability Reproducibility Stable LTP Molecular Mechanisms Synaptic Strength

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