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同时捕获实时图像在两个发射通道采用了双摄像头发射分光系统：应用到细胞粘附
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Bioengineering
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JoVE Journal Bioengineering
Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion
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同时捕获实时图像在两个发射通道采用了双摄像头发射分光系统：应用到细胞粘附

DOI:
10.3791/50604-v

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10:30 min

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September 04, 2013

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, ,
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology,Ohio University, 2Biomedical Engineering Program,Ohio University

Chapters

  • 00:05Title
  • 01:17Installation of the Dual Camera System
  • 02:47Camera Alignment
  • 04:34Preparation of Cells, Reactive Substrate, and Parallel Plate Flow Chamber
  • 05:50Two-color Image Acquisition
  • 08:27Representative Imaging Results
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

双摄像头发射分光系统，双色荧光显微镜生成具有出色的光学分辨率和时间分辨率，一定的活细胞检测的要求，包括平行板流动腔粘连检测的实时图像序列。当软件被用来从合并收购的同时发射通道的图像，伪彩色图像序列产生。

Tags

Dual Camera Emission Splitting SystemMulti-color Immunofluorescence MicroscopyParallel Plate Flow ChamberReal-time Live Cell AnalysisFluorescently Labeled CellsCell AdhesionCancer Metastasis

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