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自我报告的支架3维细胞培养
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture
DOI:

14:49 min

November 07, 2013

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Chapters

  • 00:05Title
  • 01:36Preparation and Analysis of pH Responsive Sol-gel Nanosensors
  • 04:26Preparation and Analysis of PLGA Scaffolds
  • 06:57Nanosensor Location within Mammalian Cells
  • 08:36Nanosensor Delivery to Cells Cultured upon Regular PLGA Scaffolds
  • 10:33Nanosensor Location within Mammalian Cells
  • 11:57Results: Incorporation of Nanosensors into Scaffolds and Cells
  • 14:20Conclusion

Summary

Automatic Translation

生物相容的pH响应性溶胶 - 凝胶纳米传感器可并入聚(乳酸 - 共 - 乙醇酸)(PLGA)电纺支架。所产生的自我报告的支架可用于微环境条件原位监测,同时在所述支架中培养细胞。这是有益的,因为在三维细胞构建体可以在实时而不干扰试验来监测。

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