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Selvrapportering Stilladser for 3-Dimensional Cell Culture
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture
DOI:

14:49 min

November 07, 2013

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Chapters

  • 00:05Title
  • 01:36Preparation and Analysis of pH Responsive Sol-gel Nanosensors
  • 04:26Preparation and Analysis of PLGA Scaffolds
  • 06:57Nanosensor Location within Mammalian Cells
  • 08:36Nanosensor Delivery to Cells Cultured upon Regular PLGA Scaffolds
  • 10:33Nanosensor Location within Mammalian Cells
  • 11:57Results: Incorporation of Nanosensors into Scaffolds and Cells
  • 14:20Conclusion

Summary

Automatic Translation

Biokompatible pH responsive sol-gel nanosensorer kan inkorporeres i poly (mælke-co-glycolsyre) (PLGA) Electrospun stillads. De producerede selvrapportering stilladser kan bruges til in situ overvågning af microenvironmental, men samtidig dyrkning af celler på stilladset. Dette er en fordel, da 3D cellulære konstruktion kan overvåges i realtid uden at forstyrre eksperimentet.

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