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Bioengineering
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一个微流控芯片单细胞的多功能化学分析
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Please note that all translations are automatically generated.
Click here for the English version.
一个微流控芯片单细胞的多功能化学分析
DOI:
10.3791/50618-v
•
15:41 min
•
October 15, 2013
•
Klaus Eyer
,
Phillip Kuhn
,
Simone Stratz
,
Petra S Dittrich
1
Department of Chemistry and Applied Biosciences
,
ETH Zurich, Switzerland
Chapters
00:05
Title
02:13
Fabrication of the Microfluidic Chip
05:31
Chip Preparation for Enzymatic Assays
07:15
Additional Chip Preparation for Immunoassays
10:05
Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
12:08
Results: Enzymatic and Antibody Analysis of Single Cells
15:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这篇文章中,我们提出了一个微流控芯片单细胞分析。它使细胞内的蛋白质,酶,辅因子,以及第二信使通过荧光测定法或免疫测定装置的量化。
Tags
Microfluidic Chip
Single-cell Analysis
Intracellular Biomolecules
Quantitative Determination
Cell Isolation
Cell Lysis
Lysate Retention
High Sensitivity
Array Format
Analysis Of Proteins
Enzymes
Cofactors
Second Messengers
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