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Journal
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Immunology and Infection
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建立一个<em>在体外</em>系统来研究细胞内的行为<em>光滑念珠菌</em>在人THP-1巨噬细胞
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Establishment of an
In vitro
System to Study Intracellular Behavior of
Candida glabrata
in Human THP-1 Macrophages
Please note that all translations are automatically generated.
Click here for the English version.
建立一个<em>在体外</em>系统来研究细胞内的行为<em>光滑念珠菌</em>在人THP-1巨噬细胞
DOI:
10.3791/50625-v
•
13:47 min
•
December 10, 2013
•
Maruti Nandan Rai
,
Sapan Borah
,
Gaurav Bairwa*
1
,
Sriram Balusu*
1,2
,
Neelima Gorityala
,
Rupinder Kaur
1
Laboratory of Fungal Pathogenesis
,
Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India
,
2
Current location: VIB Department for Molecular Biomedical Research, UGent
,
Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium
Chapters
00:00
Title
01:17
Preparation of THP-1 Macrophage Monolayer
02:59
Preparation of
Candida glabrata
Cell Suspension
04:26
Infection of THP-1 Macrophages with
C. glabrata
Cells
05:52
Measurement of Phagocytosis Rate and Intracellular Replication
via
Colony Forming Unit Assay
07:20
Monitoring of Intracellular Replication Using Confocal Laser Scanning Microscopy
09:07
Representative Results
09:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
当前文章概述了一个协议来建立
体外
细胞培养模型系统来研究一种兼人体细胞内的真菌病原体
光滑念珠菌
与人体巨噬细胞将是一个有用的工具,以增进我们对真菌毒力机制知识的互动。
Tags
In Vitro Model
THP-1 Macrophages
Candida Glabrata
Intracellular Pathogen
Host-pathogen Interaction
Microbial Virulence
Cell Culture
Infection Model
High-throughput Screening
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