Journal
/
Immunology and Infection
/
在FDA批准的Vero细胞疫苗开发产生重组沙粒
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
Please note that all translations are automatically generated. Click here for the English version.

在FDA批准的Vero细胞疫苗开发产生重组沙粒

DOI:
10.3791/50662-v

•

10:03 min

•

August 01, 2013

•

, , ,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Immunology and Microbial Sciences,The Scripps Research Institute

Chapters

  • 00:05Title
  • 01:36Arenavirus Rescue Transfection
  • 04:41Confirmation of Recombinant Arenavirus Rescue
  • 07:11r3LCMV and r3Candid#1 Viral Rescue
  • 08:06Results: Arenavirus in Vero Cells
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

救援重组砂粒病毒克隆的cDNA，称为反向遗传学的方法，使研究人员能够调查特定的病毒基因产物的作用，以及其不同的具体领域和残留物的贡献，砂粒病毒的生物学的许多不同方面。同样，反向遗传学技术在FDA批准的细胞系（Vero）疫苗研制的新一代安全有效的疫苗，打击人类致病的砂粒病毒提供了新的可能性。

Tags

Keywords: Recombinant ArenavirusVaccine DevelopmentFDA-approved Vero CellsArenavirus Reverse GeneticsCell-based Arenavirus Minigenome SystemsRecombinant Infectious ArenavirusesTrisegmented ArenavirusesSingle-cycle Infectious ArenavirusesReporter GenesPlasmid-based Reverse GeneticsRodent Cell LinesFDA-licensed VaccineVaccine Vectors

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50662?article_type=v

迁移人T淋巴细胞分离，培养和分析<em在体外</em
check_url/50662?article_type=v

质粒DNA重组流感病毒的一代
check_url/50662?article_type=v

使用黑色素瘤和鳞状癌干细胞生成新型支气干细胞疫苗
check_url/50662?article_type=v

使用点击化学测量的影响，病毒感染宿主细胞RNA合成
check_url/50662?article_type=v

在 MyD88的介导的细胞免疫应答西尼罗河病毒变异株感染体外分析
check_url/50662?article_type=v

利用衣壳抗原掺入战略的腺病毒血清型5载体疫苗方法的发展
check_url/50662?article_type=v

从排序的永生B细胞产生的重组人IgG单克隆抗体
check_url/50662?article_type=v

高灵敏检测方法的沙粒，细胞附着的测量
check_url/50662?article_type=v

重组呼吸道合胞病毒的生成、扩增和滴定
check_url/50662?article_type=v

体外ELISA测试评估狂犬病疫苗效力

Read Article